World's first holo-tomographic fluorescent microscope launched by Nanolive

28.03.2017 16:10

One year after launching its unique 3D Cell Explorer (the first microscope able to look inside living cells without harming them, in 3D and real time), the EPFL spin-off releases the 3D Cell Explorer-fluo. The microscopes allow scientists to monitor the effects of drugs and stimuli on virtually any kind of cell and without interfering with the normal cell physiology.

The holo-tomographic technology proper of the 3D Cell Explorer allows to measure the quantitative refractive index (RI) of the cells’ organelles with nanometric precision and instantly. The technology was published in Nature Photonics in 2013, and the 3D Cell Explorer was already awarded with multiple prestigious awards (e.g. Top 10 Innovation of 2015 from The Scientist or R&D100).

Nanolive’s newest microscope, the 3D Cell Explorer-fluo answers to the scientific need to combine the benefits from non-invasive cell tomography with a well-established and recognized method: multi-channel fluorescence microscopy. This opens completely new frontiers for research. Fluorescence microscopy offers the advantage to selectively visualize specific cell compartments by staining them with various fluorescent probes, while novel holographic tomography gives access to the whole 3D RI distribution within the cell.

This combination allows to measure, for the first time, specific cell compartments in a totally non-invasive fashion, for an unlimited amount of time and in 3D. In addition, parallel time-lapse measurements of live cells with the two technologies can be used to investigate the 4D intracellular dynamics of individual organelles. It is therefore an unrivalled solution that opens the door to new dimensions for biological investigations. Researchers can now have access to two powerful imaging modalities at the same time; they can compare and correlate resulting data and add new information to their experiments.

The potential impact of this technology for live cell research is enormous. The 3D Cell Explorer-fluoproduces results otherwise inaccessible. Some type of cells or cell organelles cannot be labeled because too delicate or very sensitive to bleaching or photo- toxicity. Up to now, these limitations hugely restrained cell analysis and quantification. Nanolive’s technology solves these problems by giving access to quantitative values of the whole cell. As a result, the 3D Cell Explorer and its fluorescence version bring a true revolution for live cell analysis. They allow scientists to monitor the effects of drugs and stimuli on virtually any kind of cell and without interfering with the normal cell physiology.

The 3D Cell Explorer-fluo is available now on the Nanolive webpage.

(Press release)

Please login or
register to comment

Please login or sign up to comment. Commenting guidelines




By continuing to use this website you agree with our TERMS AND CONDITIONS

In order to provide the best quality for you, our system uses "cookies", which are stored on your device. Cookies are necessary to identify what information (job advertisement, questionnaire, etc) you have already seen. IP address is used for the same purposes as described above.

When creating a profile, applying to the newsletter, job subscriptions and etc, you agree that the data, which you have entered, will be stored and processed in the system in order to provide services, which you have applied for.

We do NOT sell your personal data to any 3rd party services.

You must be 18 or older years old to use our services. If you are underage, you must have a permission to use our services from your parent or guardian. It is necessary in order to store and process your data.

By continuing to use our services, you agree with the these terms. You can withdraw your agreement at any time, by deleting cookies from your device and by sending request for deleting your data to the administrator.