World's first holo-tomographic fluorescent microscope launched by Nanolive

28.03.2017 16:10

One year after launching its unique 3D Cell Explorer (the first microscope able to look inside living cells without harming them, in 3D and real time), the EPFL spin-off releases the 3D Cell Explorer-fluo. The microscopes allow scientists to monitor the effects of drugs and stimuli on virtually any kind of cell and without interfering with the normal cell physiology.

The holo-tomographic technology proper of the 3D Cell Explorer allows to measure the quantitative refractive index (RI) of the cells’ organelles with nanometric precision and instantly. The technology was published in Nature Photonics in 2013, and the 3D Cell Explorer was already awarded with multiple prestigious awards (e.g. Top 10 Innovation of 2015 from The Scientist or R&D100).

Nanolive’s newest microscope, the 3D Cell Explorer-fluo answers to the scientific need to combine the benefits from non-invasive cell tomography with a well-established and recognized method: multi-channel fluorescence microscopy. This opens completely new frontiers for research. Fluorescence microscopy offers the advantage to selectively visualize specific cell compartments by staining them with various fluorescent probes, while novel holographic tomography gives access to the whole 3D RI distribution within the cell.

This combination allows to measure, for the first time, specific cell compartments in a totally non-invasive fashion, for an unlimited amount of time and in 3D. In addition, parallel time-lapse measurements of live cells with the two technologies can be used to investigate the 4D intracellular dynamics of individual organelles. It is therefore an unrivalled solution that opens the door to new dimensions for biological investigations. Researchers can now have access to two powerful imaging modalities at the same time; they can compare and correlate resulting data and add new information to their experiments.

The potential impact of this technology for live cell research is enormous. The 3D Cell Explorer-fluoproduces results otherwise inaccessible. Some type of cells or cell organelles cannot be labeled because too delicate or very sensitive to bleaching or photo- toxicity. Up to now, these limitations hugely restrained cell analysis and quantification. Nanolive’s technology solves these problems by giving access to quantitative values of the whole cell. As a result, the 3D Cell Explorer and its fluorescence version bring a true revolution for live cell analysis. They allow scientists to monitor the effects of drugs and stimuli on virtually any kind of cell and without interfering with the normal cell physiology.

The 3D Cell Explorer-fluo is available now on the Nanolive webpage.

(Press release)

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